Initial adhesion of bone marrow stromal cells to various bone graft substitutes
조영재, 김경화, 구기태, 김태일, 설양조, 이용무, 구영, 정종평, 류인철,
소속 상세정보
조영재 ( Jo Young-Jae ) - Seoul National University College of Medicine Department of Periodontology
김경화 ( Kim Kyoung-Hwa ) - Seoul National University College of Medicine Department of Periodontology
구기태 ( Koo Ki-Tae ) - Seoul National University College of Medicine Department of Periodontology
김태일 ( Kim Tae-Il ) - Seoul National University College of Medicine Department of Periodontology
설양조 ( Seol Yang-Jo ) - Seoul National University College of Medicine Department of Periodontology
이용무 ( Lee Yong-Moo ) - Seoul National University College of Medicine Department of Periodontology
구영 ( Ku Young ) - Seoul National University College of Medicine Department of Periodontology
정종평 ( Chung Chong-Pyoung ) - Seoul National University College of Medicine Department of Periodontology
류인철 ( Rhyu In-Chul ) - Seoul National University College of Medicine Department of Periodontology
KMID : 0363020110410020067
Abstract
Purpose: The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro.
Methods: Rat bone marrow derived stromal cells were used. The biomaterials were deproteinized bovine bone mineral (DBBM), DBBM coated with fibronectin (FN), synthetic hydroxyapatite (HA), HA coated with FN, HA coated with β-tricalcium phosphate (TCP), and pure β-TCP. With confocal laser scanning microscopy, actin filaments and vinculin were observed after 6, 12, and 24 hours of cell seeding. The morphological features of cells on each biomaterial were observed using scanning electron microscopy at day 1 and 7.
Results: The cells on HA/FN and HA spread widely and showed better defined actin cytoskeletons than those on the other biomaterials. At the initial phase, FN seemed to have a favorable effect on cell adhesion. In DBBM, very few cells adhered to the surface.
Conclusions: Within the limitations of this study, we can conclude that in contrast with DBBM not supporting cell attachment, HA provided a more favorable environment with respect to cell attachment.
키워드
Bone substitutes; Cell adhesion; Fibronectins; Stem cells
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